Delichon Biotechnology Ltd. FINLAND

Antiserum to GABA Lot No 3001B

Product number 3001-1 Antiserum to GABA Lot No 3001-C

Immunization Protocol

Immunogen: GABA coupled to keyhole limpet hemocyanin
Animal species: Rabbit
Immunization: Multiple intradermal injections

Applications and Specificity

Applications: Immunocytochemistry of GABA-containing cells and tissues in different animal species. The optimal dilution depends on the tissue, fixation method and detection system. With immunofluorescence method the optimal dilution is 1:500- 1:1 000. The optimal dilution should be tested in each system with serial dilutions (e.g.1:500, 1:1000, 1:2000, 1:5000, 1:10 000, 1:20 000, 1:50 000) for best results. The antiserum gives high-quality staining in tissues of molluscs, mouse and rat. The antiserum requires fixation of the tissues with 1-ethyl-3(3-dimethylaminopropyl)carbodiimide (EDAC). The antiserum is not suitable for samples fixed with aldehydes only.

This antiserum is sold as a reagent for research purposes only. Not for in vitro diagnostic use. It is not licensed or approved for adminstration to humans.

Specificity: The specificity of the antiserum has been tested on nitrocellulose filters containing amino acid-protein and histamine-protein conjugates (in the mammalian brain GABA and histamine coexist in the same cells of hypothalamic tuberomamilary nucleus). The antiserum detects specifically GABA conjugated to proteins with EDAC.

Storage: Undiluted antiserum can be stored at -70 C. Lyophilized antiserum can be reconstituted by adding the given amount of sterile water. Repeated freezing and thawing should be avoided. For practical reasons, we recommend that the users divide the antiserum in aliquots which are kept frozen. Glycerol (50%) can be added to prevent repeated freezing.


Polak J, Kendall PA, Heath CM, Pearse AGE (1972) Carbodiimide fixation for electron microscopy and immunoelectron microscopy. Experientia 28: 368-369.

Panula P, Yang HYT, Costa E (1984) Histamine-containing neurons in the rat hypothalamus. Proc. Natl. Acad. Sci. USA 81: 2572-2576.

Karhunen T and Panula P (1993) Neurotransmitters in the nervous system of Macoma balthica (Bivalvia). J.Comp. Neurol. 334: 477-488.